RESUMO
Localization of virus RNA in stroma of Capsicum anuum L. chloroplasts was determined by the PCR method. Accumulation of virus protein in the membranes and stroma of infected pepper chloroplasts has been studied. It is concluded that the virus protein synthesis takes place in the pepper chloroplasts.
Assuntos
Capsicum/virologia , Cloroplastos/virologia , Reação em Cadeia da Polimerase/métodos , RNA Viral/ultraestrutura , Capsicum/anatomia & histologia , Cloroplastos/patologia , Cloroplastos/ultraestrutura , Vírus do Mosaico do Tabaco/ultraestruturaRESUMO
Chlorotic areas of Capsicuum anuum L. leaves infected with tobacco mosaic virus (TMV) have been investigated. Pathological changes of chloroplasts were found out. Swelling, more osmophilic plastoglobuli, loosened thylakoid structure were observed. It was shown that chloroplasts did not take part in the process of virus replication. Some structural changes of mitochondria were discovered too. They were most tolerant to the virus infection in comparison with other organoids.